This talk was recorded at the 2017 Single Cell Analyses Meeting at the Cold Spring Harbor Laboratory. It summarizes results published in: Budnik B., Levy E., Slavov N. (2017) Mass-spectrometry of single mammalian cells quantifies proteome heterogeneity during cell differentiation, bioRxiv
the Slavov laboratory has developed Single Cell ProtEomics by Mass Spectrometry (SCoPE-MS) and uses SCoPE-MS for measuring proteome configurations of single cells and linking them to functional phenotypes, such as cell type and differentiation potentials, as well as for studying post-transcriptional regulation in single cells.
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I am looking forward to the Festival of Genomics in Boston !
Author summary Hematopoietic stem cells are classically defined as a specific category of cells at the top of the hierarchy that can differentiate all blood cell types following step-by-step the instructions of a deterministic program. We have analysed this process, and our findings support a much more dynamic view than previously described. We apply time-lapse microscopy coupled to single-cell molecular analyses in human hematopoietic stem cells and find that fate decision is not a unique, programmed event but a process of spontaneous variation and selective stabilisation reminiscent of trial–error processes. We show that each cell explores (at its own pace and independently of cell division) many different possibilities before reaching a stable combination of genes to be expressed. Our results suggest, therefore, that multipotency seems to be more like a transitory state than a feature of a specific cell category.
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This is one of those simple and elegant solutions that will immediately make you think “oh…why the Albert Heck didn’t I think of that…?!?!”
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Quantifying proteins in single cells directly, without relying on antibodies, has been a long standing aim and dream for many scientists.
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